Journal
MOLECULAR PHARMACEUTICS
Volume 10, Issue 3, Pages 1008-1019Publisher
AMER CHEMICAL SOC
DOI: 10.1021/mp300453k
Keywords
sodium taurocholate cotransporting polypeptide (NTCP); apical sodium dependent bile acid transporter (ASBT); pharmacophore; Bayesian; transporter
Funding
- National Institutes of Health [DK093406]
- FDA [U01FD004320-01]
- FDA [1U01FD004320-01, 544118] Funding Source: Federal RePORTER
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The hepatic bile acid uptake transporter sodium taurocholate cotransporting polypeptide (NTCP) is less well characterized than its ileal paralog, the apical sodium dependent bile acid transporter (ASBT), in terms of drug inhibition requirements. The objectives of this study were (a) to identify FDA approved drugs that inhibit human NTCP, (b) to develop pharmacophore and Bayesian computational models for NTCP inhibition, and (c) to compare NTCP and ASBT transport inhibition requirements. A series of NTCP inhibition studies were performed using FDA approved drugs, in concert with iterative computational model development. Screening studies identified 27 drugs as novel NTCP inhibitors, including irbesartan (K-i = 11.9 mu M) and ezetimibe (K-i = 25.0 mu M). The common feature pharmacophore indicated that two hydrophobes and one hydrogen bond acceptor were important for inhibition of NTCP. From 72 drugs screened in vitro, a total of 31 drugs inhibited NTCP, while Si drugs (i.e., more than half) inhibited ASBT. Hence, while there was inhibitor overlap, ASBT unexpectedly was more permissive to drug inhibition than was NTCP, and this may be related to NTCP possessing fewer pharmacophore features. Findings reflected that a combination of computational and in vitro approaches enriched the understanding of these poorly characterized transporters and yielded additional chemical probes for possible drug transporter interaction determinations.
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