Journal
MOLECULAR PHARMACEUTICS
Volume 11, Issue 7, Pages 2213-2223Publisher
AMER CHEMICAL SOC
DOI: 10.1021/mp400269z
Keywords
methotrexate; chitosan; mPEG; cellular uptake; targeted delivery
Funding
- key jointed Foundation of the National Science Foundation of China-Yunnan [U1137601]
Ask authors/readers for more resources
Cancer nanotherapeutics are rapidly progressing and being implemented to solve several limitations of conventional drug delivery systems. In this paper, we report a novel strategy of preparing methotrexate (MTX) nanopartides based on chitosan (CS) and methoxypoly(ethylene glycol) (mPEG) used as nanocarriers to enhance their targeting and prolong blood circulation. MTX and mPEG-conjugated CS nanopartides (NPs) were prepared and evaluated for their targeting efficiency and toxicity in vitro and in vivo. The MTX-mPEG-CS NP size determined by dynamic light scattering was 213 +/- 2.0 nm with a narrow particle size distribution, and its loading content (LC %) and encapsulation efficiency (EE) were 44.19 +/- 0.64% and 87.65 +/- 0.79%, respectively. In vitro release behavior of MTX was investigated. In vivo optical imaging in mice proved that MTX was released from particles subsequently and targeted to tumor tissue, showing significantly prolonged retention and specific selectivity. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay obviously indicated that the higher inhibition efficiency of MTX-mPEG-CS NPs meant that much more MTX was transferred into the tumor cells. A significant right-shift in the flow cytometry (FCM) assay demonstrated that MTX-loaded nanopartides were far superior to a pure drug in the inhibition of growth and proliferation of Hela cells. These results suggest that MTX mPEG CS NPs could be a promising targeting anticancer chemotherapeutic agent, especially for cervical carcinoma.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available