Journal
MOLECULAR PHARMACEUTICS
Volume 5, Issue 5, Pages 898-901Publisher
AMER CHEMICAL SOC
DOI: 10.1021/mp800094b
Keywords
carboxymethyl poly(L-histidine); pH-sensitive polypeptide; polyplex; gene delivery
Funding
- Japan Science and Technology Agency [05-037]
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Carboxymethyl poly(L-histidine) (CM-PLH) as a new pH-sensitive polypeptide has enhanced polyplex gene delivery. Agarose gel retardation assay and zeta potential measurement proved that the anionic CM-PLH at physiological pH coated the PEI/DNA binary complexes. The resulting CM-PLH/PEI/DNA ternary complexes showed the gene expression value 300 times higher than that of the PEI/DNA binary complexes. These results suggest that the synergistic effect of the pH-sensitive imidazole groups at endosomal pH and the anionic carboxymethyl groups at physiological pH in the CM-PLH enhanced polyplex gene delivery.
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