Journal
MOLECULAR PHARMACEUTICS
Volume 5, Issue 2, Pages 287-293Publisher
AMER CHEMICAL SOC
DOI: 10.1021/mp7001158
Keywords
liposomes; Raman imaging; drug delivery; cells
Funding
- NCI NIH HHS [R01 CA090346, CA 090346, R01 CA090346-05A1, R01 CA121838, CA121838] Funding Source: Medline
- NHLBI NIH HHS [HL55519, R01 HL055519] Funding Source: Medline
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Recent developments, combining Raman spectroscopy with optical microscopy, provide a new noninvasive technique to assess and image cellular processes. Of particular interest are the uptake mechanisms of various cytologically active compounds. In order to distinguish the species of interest from their cellular environment spectroscopically, compounds may be labeled With deuterium. Here, we apply Raman microspectroscopy to follow the uptake of liposomal drug carrier systems that have been introduced to deliver biologically active compounds to their site of action within human breast adenocarcinoma MCF-7 cells. The distribution patterns of liposomes and liposomes surface-modified with a cell-penetrating peptide (TAT-peptide, TATp) have been imaged over time. The spectroscopic information obtained provides a clear evidence for variable rates, as well as different efficiencies of liposome uptake depending on their surface properties. Depending on the experimental setup, the technique may be applied to fixed or living cell organisms.
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