Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 58, Issue 7, Pages 1436-1446Publisher
WILEY-BLACKWELL
DOI: 10.1002/mnfr.201300763
Keywords
3T3-L1; 18 beta-glycyrrhetinic acid; Cannabinoid receptor type 1; Licorice; Obesity
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Funding
- National Research Foundation, Korea [2012R1A2A1A03006092]
- National Research Foundation of Korea [2012R1A2A1A03006092] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Scope: Previous reports suggest that licorice extract has various metabolically beneficial effects and may help to alleviate adiposity and hyperlipidemia. However, underlying anti-obesity mechanisms still remain elusive. Moreover, it is unknown which single ingredient in licorice extract would mediate such effects. We aimed to demonstrate that licorice extract and its active ingredients can inhibit adipocyte differentiation and fat accumulation. Methods and results: 18 beta-glycyrrhetinic acid (18 beta-GA) alleviated the effects of CB1R agonist, anandamide (AEA) on CB1R signaling in a concentration-dependent manner. Consistently, 18 beta-GA suppressed AEA-induced adipocyte differentiation in 3T3-L1 cells through the downregulation of AEA-induced MAPK activation and expression of adipogenic genes including C/EBP-alpha and PPAR-gamma. The protein levels of fatty acid synthase and stearoyl-CoA desaturase 1 were also decreased and the phosphorylation of acetyl-CoA carboxylase was increased in 18 beta-GA pretreated cells. The supplementation of 18 beta-GA significantly lowered body weight, fat weight, and plasma lipids levels in obese animal models. Conclusion: These results may provide a novel insight into the molecular mechanism involved in anti-adipogenic and anti-obesity effects of 18 beta-GA by suppressing the activation of CB1R induced by AEA. Thus, 18 beta-GA may exert beneficial effects against obesity-related metabolic disorders.
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