Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 58, Issue 11, Pages 2166-2176Publisher
WILEY-BLACKWELL
DOI: 10.1002/mnfr.201400159
Keywords
Adipogenesis; Galanin-mediated signaling; Mice; Oleuropein; WNT10b-mediated signaling
Categories
Funding
- SRC program (Center for Food & Nutritional Genomics) [2008-0062618]
- Technology Commercialization Support Program, Ministry of Agriculture, Food and Rural Affairs [1130373]
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Scope: The aim of the present study was to investigate the antiobesity effect of oleuropein on high-fat diet (HFD) induced body weight gain and visceral adiposity in mice, and to explore the underlying mechanisms involved. Methods and results: C57BL/6N mice were fed with a normal diet, HFD (40% fat of total energy), and HFD-supplemented with 0.03% oleuropein for 10 wk. Oleuropein significantly reduced HFD-induced body weight gain and visceral adiposity. Oleuropein also significantly reversed the HFD-induced elevations of adipogenic related gene expression involved in WNT10b-and galanin-mediated signalings in adipose tissue of mice. Consistent with in vivo findings, oleuropein dose-dependently suppressed lipid accumulation in 3T3-L1 cells during preadipocyte differentiation. Additionally, exposure of the 3T3-L1 preadipocytes to oleuropein resulted in a marked attenuation of the secreted frizzled-related protein 2 (WNT inhibitor) or galnon (galanin receptor agonist) induced cellular lipid accumulation. Conclusion: This study demonstrated the oleuropein-reduced body weight gain and visceral adiposity in HFD-fed mice. The protective effect of oleuropein against HFD-induced adiposity in mice appeared to be mediated through the upregulation of genes involved in WNT10b-mediated signaling and downregulation of genes involved in galanin-mediated signaling cascades.
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