Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 58, Issue 11, Pages 2177-2188Publisher
WILEY
DOI: 10.1002/mnfr.201300901
Keywords
Dietary fats; Glucose metabolism disorders; Insulin resistance; Metabolic syndrome; n-3 PUFA
Categories
Funding
- European Union [505944]
- Ministerio de Economia y Competitividad [AGL2009-12270, AGL2012-39615, BFU2010-17116]
- CIBERobn [CB06/03/0047]
- Consejeria de Innovacion, Ciencia y Empresa, Junta de Andalucia [P06-CTS-01425, CVI-7450, CTS-03039, CTS-6606, BIO-0139, CTS-5015]
- Consejeria de Salud, Junta de Andalucia [06/128, 07/43, PI-0193, PI-0058/10, PI-0252/09]
- Fondo Europeo de Desarrollo Regional (FEDER)
- SFI PI Programme [11/PI/1119]
- Instituto de Salud Carlos III, Spain [CD07/00208]
- Genoma Espana
Ask authors/readers for more resources
Scope: To determine whether the insulin resistance that exists in metabolic syndrome (MetS) patients is modulated by dietary fat composition. Methods and results: Seventy-five patients were randomly assigned to one of four diets for 12 wk: high-saturated fatty acids (HSFAs), high-MUFA (HMUFA), and two low-fat, high-complex carbohydrate (LFHCC) diets supplemented with long-chain n-3 (LFHCC n-3) PUFA or placebo. At the end of intervention, the LFHCC n-3 diet reduced plasma insulin, homeostasis model assessment of insulin resistance, and nonsterified fatty acid concentration (p < 0.05) as compared to baseline Spanish habitual (BSH) diet. Subcutaneous white adipose tissue (WAT) analysis revealed decreased EH-domain containing-2 mRNA levels and increased cbl-associated protein gene expression with the LFHCC n-3 compared to HSFA and HMUFA diets, respectively (p < 0.05). Moreover, the LFHCC n-3 decreased gene expression of glyceraldehyde-3-phosphate dehydrogenase with respect to HMUFA and BSH diets (p < 0.05). Finally, proteomic characterization of subcutaneous WAT identified three proteins of glucose metabolism downregulated by the LFHCC n-3 diet, including annexin A2. RT-PCR analysis confirmed the decrease of annexin A2 (p = 0.027) after this diet. Conclusion: Our data suggest that the LFHCC n-3 diet reduces systemic insulin resistance and improves insulin signaling in subcutaneous WAT of MetS patients compared to HSFA and BSH diets consumption.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available