Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 55, Issue -, Pages S117-S126Publisher
WILEY
DOI: 10.1002/mnfr.201000220
Keywords
Breast Cancer; DMBA; Hydroxytyrosol; Microarray; Rat
Categories
Funding
- Excelentisima Diputacion de Jaen
- CEAS Foundation [30.C0.244500]
- Junta de Andalucia [PI-0210/2007]
- Spanish Ministry of Science and Innovation [AP2005-144]
Ask authors/readers for more resources
Scope: Hydroxytyrosol (a phenolic compound derived from virgin olive oil) has demonstrated an anti-tumour effect in several tumour cell lines in addition to other health-related properties. The aim of this study was to investigate, for the first time in an animal model of experimental mammary carcinoma, the anti-cancer ability of hydroxytyrosol and to discover which pathways are modified by hydroxytyrosol. Methods and results: Dimethylbenz[a] anthracene-induced mammary tumours were induced in 28 female Sprague-Dawley rats and ten of them were treated with hydroxytyrosol (0.5 mg/kg b.w. 5 days/week for 6 wk). cDNA microarray and quantitative RT-PCR experiments were performed. Hydroxytyrosol was found to inhibit the experimental mammary tumour growth and proliferation rate, with results comparable to those of doxorubicin but better with regard to the histopathological outcome. It also altered the expression of genes related to apoptosis, cell cycle, proliferation, differentiation, survival and transformation pathways. Conclusions: This study shows that hydroxytyrosol exerts anti-cancer effects in Sprague-Dawley rats with experimental mammary tumours, inhibiting growth and cell proliferation in mammary tumours. Moreover, hydroxytyrosol alters several genes associated with cell proliferation, apoptosis and the Wnt signalling pathway, promoting a high expression of Sfrp4. However, further studies are necessary to better understand the mechanisms of hydroxytyrosol.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available