Journal
MOLECULAR NEURODEGENERATION
Volume 6, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1750-1326-6-38
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Funding
- National Program of Basic Research of China [2007CB947900, 2010CB945200, 2011CB504104]
- Natural Science Fund [30700888, 30770732, 30872729, 30971031]
- Key Discipline Program of Shanghai Municipality [S30202]
- Shanghai Key Project of Basic Science Research [10411954500]
- Shanghai Pujiang Program [08PJ1407900]
- Program for Outstanding Medical Academic Leader of Shanghai [LJ 06003]
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Background: Abnormal accumulation and aggregation of microtubule associated proteins (MAPs) plays an important role in the pathogenesis of neurodegenerative diseases. Loss-of-function mutation of DJ-1/Park7 can cause early onset of PD. DJ-1, a molecular chaperone, can inhibit a-synuclein aggregation. Currently, little is known whether or not loss of function of DJ-1 contributes to abnormal MAPs aggregation in neurodegenerative disorders such as PD. Results: We presented evidence that DJ-1 could bind to microtubule associated protein1b Light Chain (MAP1b-LC). Overexpression of DJ-1 prevented MAP1b-LC aggregation in HEK293t and SH-SY5Y cells while DJ-1 knocking down (KD) enhanced MAP1b-LC aggregation in SH-SY5Y cells. The increase in insoluble MAP1b-LC was also observed in the DJ-1 null mice brain. Moreover, in the DJ-1 KD SH-SY5Y cells, overexpression of MAP1B-LC led to endoplasmic reticulum (ER) stress-induced apoptosis. Conclusion: Our results suggest that DJ-1 acts as a molecular chaperone to inhibit MAP1B aggregation thus leading to neuronal apoptosis. Our study provides a novel insight into the mechanisms that underly the pathogenesis of Parkinson's disease (PD).
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