Journal
MOLECULAR NEUROBIOLOGY
Volume 53, Issue 1, Pages 28-39Publisher
HUMANA PRESS INC
DOI: 10.1007/s12035-014-8968-2
Keywords
Biomaterials; Polysaccharides; miRNA; Oligosaccharides; Regeneration
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Funding
- National Natural Science Foundation of China [81371687, 81171457, 81130080, 31471011]
- Ministry of Science and Technology of China Grants [2014CB542202, 2012AA020502]
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
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Natural polysaccharides are biomaterials widely used for constructing scaffolds in tissue engineering. While natural polysaccharides have been shown to robustly promote tissue regeneration, the underlying molecular mechanism remains largely unknown. Here, we show that chitooligosaccharides (COS), the intermediate products of chitosan degradation, stimulate peripheral nerve regeneration in rats. Our experiment also shows that COS stimulate the proliferation of Schwann cells (SCs) during nerve regeneration. By analyzing the transcriptome and gene regulatory network, we identified the miR-27a/FOXO1 axis as the main signaling pathway for mediating the proliferative effects of COS on SCs. COS increase the expression level of miR-27a and cause a reduction of FOXO1, which subsequently accelerates the cell cycle and stimulates SC proliferation to stimulate nerve regeneration. These findings define a basic pathway for oligosaccharides-mediated cell proliferation and reveal a novel aspect of polysaccharide biomaterials in tissue engineering.
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