Journal
MOLECULAR NEUROBIOLOGY
Volume 47, Issue 3, Pages 976-987Publisher
SPRINGER
DOI: 10.1007/s12035-013-8396-8
Keywords
Tube formation; Oxygen-glucose deprivation; Sonic hedgehog; RhoA; Brain microvascular endothelial cells
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Funding
- National Natural Science Foundation of China [81070938, 81101905]
- New Century Excellent Talents in University [NCET-10-0406]
- Fundamental Research Funds for the Central Universities, HUST [2010JC028]
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The human adult brain possesses intriguing plasticity, including neurogenesis and angiogenesis, which may be mediated by the activated sonic hedgehog (Shh). By employing a coculture system, brain microvascular endothelial cells (BMECs) cocultured with astrocytes, which were incubated under oxygen-glucose deprivation (OGD) condition, we tested the hypothesis that Shh secreted by OGD-activated astrocytes promotes cerebral angiogenesis following ischemia. The results of this study demonstrated that Shh was mainly secreted by astrocytes and the secretion was significantly upregulated after OGD. The proliferation, migration, and tube formation of BMECs cocultured with astrocytes after OGD were significantly enhanced, but cyclopamine (a Shh antagonist) or 5E1 (an antibody of Shh) reversed the change. Furthermore, silencing Ras homolog gene family, member A (RhoA) of BMECs by RNAi and blocking Rho-dependent kinase (ROCK) by Y27632, a specific antagonist of ROCK, suppressed the upregulation of proliferation, migration, and tube formation of BMECs after OGD. These findings suggested that Shh derived from activated astrocytes stimulated RhoA/ROCK pathway in BMECs after OGD, which might be involved in angiogenesis in vitro.
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