Journal
MOLECULAR MICROBIOLOGY
Volume 87, Issue 4, Pages 756-768Publisher
WILEY
DOI: 10.1111/mmi.12129
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Funding
- Natural Sciences and Engineering Research Council of Canada
- Ministere du Developpement economique, de l'Innovation et de l'Exportation, Programme de soutien a la recherche: Programme de soutien a des initiatives internationales de recherche et d'innovation
- Fonds Quebecois de Recherche sur la Nature et les Technologies
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AbiQ is a phage resistance mechanism found on a native plasmid of Lactococcus lactis that abort virulent phage infections. In this study, we experimentally demonstrate that AbiQ belongs to the recently described type III toxinantitoxin systems. When overexpressed, the AbiQ protein (ABIQ) is toxic and causes bacterial death in a bacteriostatic manner. Northern and Western blot experiments revealed that the abiQ gene is transcribed and translated constitutively, and its expression is not activated by a phage product. ABIQ is an endoribonuclease that specifically cleaves its cognate antitoxin RNA molecule in vivo. The crystal structure of ABIQ was solved and site-directed mutagenesis identified key amino acids for its anti-phage and/or its RNase function. The AbiQ system is the first lactococcal abortive infection system characterized to date at a structural level.
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