Journal
MOLECULAR MICROBIOLOGY
Volume 79, Issue 1, Pages 109-118Publisher
WILEY
DOI: 10.1111/j.1365-2958.2010.07433.x
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Funding
- National Institute of Health [RO1GM081567]
- Takara Bio
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM081567] Funding Source: NIH RePORTER
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Toxin-antitoxin (TA) systems of free-living bacteria have recently demonstrated that these toxins inhibit cell growth by targeting essential functions of cellular metabolism. Here we show that YeeV toxin inhibits cell division, leads to a change in morphology and lysis of Escherichia coli cells. YeeV interacts with two essential cytoskeleton proteins, FtsZ and MreB. Purified YeeV inhibits both the GTPase activity and the GTP-dependent polymerization of FtsZ. YeeV also inhibits ATP-dependent polymerization of MreB. Truncated C-terminal deletions of YeeV result in elongation of cells, and a deletion of the first 15 amino acids from the N-terminus of YeeV caused lemon-shaped cell formation. The YeeV toxin is distinct from other well-studied toxins: it directs the binding of two cytoskeletal proteins and inhibits FtsZ and MreB simultaneously.
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