Journal
MOLECULAR MICROBIOLOGY
Volume 69, Issue 4, Pages 954-967Publisher
WILEY
DOI: 10.1111/j.1365-2958.2008.06331.x
Keywords
-
Categories
Funding
- National Institutes of Health [GM-047446]
Ask authors/readers for more resources
We have investigated the function of a cell envelope stress-inducible gene, yvrl, which encodes a 22.5 kDa protein that includes a predicted sigma(70) region 4 domain, but lacks an apparent region 2 domain. Yvrl interacts with RNA polymerase and overexpression of Yvrl results in induction of OxdC, an oxalate decarboxylase maximally expressed under low-pH conditions. We have used microarray-based analyses to define the YvrI regulon. Yvrl is required for the transcription of three operons (oxdC-yvrL, yvrJ and yvrI-yvrHa) each of which is preceded by a highly similar promoter sequence. Activation of these promoters requires both YvrI and the product of the second gene in the yvrI-yvrHa operon, YvrHa. YvrI and YvrHa together allow recognition of the oxdC promoter, stimulate DNA melting and activate transcription by core RNA polymerase. Together, these results suggest that Yvrl is a previously unrecognized a factor in Bacillus subtilis and that the 9.5 kDa YvrHa protein acts as a required co-activator of transcription. A yvrL deletion results in the upregulation of YvrI activity suggesting that YvrL is a negative regulator of YvrI-dependent transcription, possibly functioning as an anti-sigma factor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available