Journal
MOLECULAR MEDICINE REPORTS
Volume 10, Issue 5, Pages 2511-2516Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2014.2557
Keywords
iTRAQ; hyperhomocysteinemia; pathway analysis; tau; Camk2
Categories
Funding
- National Natural Science Foundation of China [81171163]
- Key Projects of Science and Technology Commission of Shanghai [11411952100]
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High levels of homocysteine, caused by abnormal methionine metabolism, can induce degeneration of mouse hippocampal neurons. iTRAQ (TM) technology has been widely used in the field of proteomics research and through employing this technology, the present study identified that hyperhomocysteinemia induced the downregulation of 52 proteins and upregulation of 44 proteins in the mouse hippocampus. Through gene ontology and pathway analysis, the upregulation of components of the cytoskeleton, actin, regulators of focal adhesion, calcium signaling pathways, tight junctions, ErbB and gonadotrophin-releasing hormone signaling, leukocyte, transendothelial migration, propanoate and pyruvate metabolism, valine, leucine and isoleucine biosynthesis, synthesis and degradation of ketone bodies and benzoate degradation via CoA ligation pathway, was identified. It was additionally verified that tau protein was highly expressed in the hyperhomocysteinemic neurons. Further analysis revealed that tau network proteins played functional roles in homocysteine-induced neuronal damage.
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