Journal
MOLECULAR MEDICINE REPORTS
Volume 7, Issue 3, Pages 1045-1049Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2013.1290
Keywords
tanshinone IIA; BxPC-3 cells; TCTP; MCL-1
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Funding
- Research Section of the Changhua Christian Hospital (Changhua, Taiwan) [101-CCH-IRP-11]
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Pancreatic cancer remains a challenging disease worldwide. Tanshinone IIA (Tan-IIA) is one of the active constituents of Danshen (Radix Salviae miltiorrhizae). Tan-IIA has been hypothesized to inhibit numerous human cancer cells by various molecular mechanisms. However, the efficacy and molecular mechanism of Tan-IIA action in pancreatic cancer has not been well studied. In the present study, the cytotoxicity of Tan-IIA in human pancreatic cancer BxPC-3 cells was evaluated by MTT assay. Cell cycle analysis of BxPC-3 cells treated with Tan-IIA was performed by flow cytometry (FACS). Protein expression levels of TCTP, MCL-1, Bcl-xL, Bax and Caspase-3 in BxPC-3 cells were measured by western blot analysis. The results revealed that Tan-IIA inhibited BxPC-3 cells in a time- and dose-dependent manner. FACS analysis demonstrated that Tan-IIA increases the rate of sub-G, phase. BxPC-3 cells treated with Tan-IIA were identified to upregulate protein expression of Bax and Caspase-3 and downregulate expression of TCTP, MCL-1 and Bcl-xL. These results indicate that Tan-IIA may inhibit BxPC-3 human pancreatic cancer cells through the induction of apoptosis by decreasing protein expression of TCTP, MCL-1 and Bcl-xL and increasing Bax expression in vitro. The chemotherapeutic potential of Tan-IIA for human pancreatic cancer warrants further study.
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