Journal
MOLECULAR IMMUNOLOGY
Volume 48, Issue 12-13, Pages 1432-1438Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2011.03.022
Keywords
beta-Defensin; Antimicrobial peptide; Real-time PCR; Chemotaxis; Teleost fish; Gilthead seabream
Categories
Funding
- regional Fundacion Seneca [04538/GERM/06]
- national Ministerio de Ciencia e Innovacion [AGL2008-05119-C02-01]
- Ramon y Cajal
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Antimicrobial peptides (AMPs) are important mediators of the innate immune response against bacteria and viruses. We have found a beta-defensin (BD) gene searching the expressed sequence tags (ESTs) of the teleost fish gilthead seabream (Sparus aurata). The clone contains an open reading frame of 201 bp mRNA that encodes a putative seabream beta-defensin (saBD) propeptide of 66 amino acids containing the six conserved cysteines as the main signature of this AMP. The phylogenetic tree shows that saBD, and its fish orthologues, are closely related to the human BD-4. Transcripts of the saBD gene were mainly detected by real-time PCR in the skin, peritoneal leucocytes and head-kidney but scarcely expressed in the peripheral blood. Interestingly, head-kidney leucocytes incubation with synthetic unmethylated CpG oligodeoxynucleotides and bacterial DNA up-regulated the saBD gene expression. Recombinant protein (saBD-V5-His) was expressed in the HEK293 cell line and its functional activity determined. First, seabream head-kidney leucocytes showed chemotactic activity towards supernatants containing saBD-V5-His whilst failed to do so to human recombinant BD-1 y BD-4. Moreover, both cell lysates and supernatants containing saBD-V5-His showed strong antimicrobial activity against Vibrio anguillarum (a seabream pathogenic bacterium) and Bacillus subtilis whilst little on other fish pathogens such as Vibrio harvey and Photobacterium damselae. Further studies will elucidate the existence of other BD genes and their implications on the seabream defense against bacteria and virus. (C) 2011 Elsevier Ltd. All rights reserved.
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