Journal
MOLECULAR IMMUNOLOGY
Volume 46, Issue 13, Pages 2548-2556Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2009.05.012
Keywords
Common carp; Type I interferon; Cloning; Structural analysis; mRNA expression
Categories
Funding
- Japan Society for the Promotion of Science (JSPS) [20580206, 21780180]
- Grants-in-Aid for Scientific Research [21780180, 20580206] Funding Source: KAKEN
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The type I interferon (I-IFN) gene has recently been cloned and sequenced in the common carp species Cyprinus carpio L Carp I-IFN cDNA is composed of 675 base pairs and is translated into a protein of 186 amino acid residues. The carp I-IFN encodes a predicted signal peptide of 23 amino acid residues and contains the I-IFN family signature His(140)-Trp(158). Analysis of the homology between carp I-IFN and other known I-IFN and type II interferon (II-IFN) family members has revealed significant similarities to grass carp I-IFN. Phylogenetic analysis demonstrated that carp I-IFN clusters with I-IFN in teleosts, away from the other II-IFN family members. In addition, the gene structure for carp I-IFN is composed of 5 exons and 4 introns, a composition that is similar to that of the teleost I-IFN gene. RT-PCR analysis did not reveal gene expression in un-stimulated tissues including intestine, liver, gill, head kidney, muscle, spleen, mid-kidney and skin. However, I-IFN expression levels increased following stimulation with imiquimod in the head kidney cells. Furthermore, recombinant carp I-IFN protein (mature form) produced via the cell-free protein synthesis system stimulated the expression of the interferon-inducible Mx gene in the head kidney cells. (C) 2009 Elsevier Ltd. All rights reserved.
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