Journal
MOLECULAR IMAGING AND BIOLOGY
Volume 17, Issue 1, Pages 38-48Publisher
SPRINGER
DOI: 10.1007/s11307-014-0763-y
Keywords
MUC1 aptamer; Tumor targeting; PEG; Near-infrared fluorescence; Tumor imaging
Funding
- Natural Science Foundation Committee of China (NSFC) [61335007, 81371684, 81220108012, 81000666, 81171395, 81328012]
- Project Program of State Key Laboratory of Natural Medicines, China Pharmaceutical University - Priority Academic Program Development of Jiangsu Higher Education Institutions [SKLNMZZYQ201403]
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Purpose: DNA aptamer (APT) is able to bind to Mucin 1 (MUC1) specifically. The possibility of APT acting as a moiety to construct tumor-targeting probes was investigated. Procedures: A near-infrared (NIR) fluorescent dye (MPA) and polyethylene glycol (PEG) were conjugated to APT to form APT-MPA and APT-PEG-MPA. The successful synthesis of the two probes was characterized via thin layer chromatography (TLC) and optical spectra. The tumor-targeting efficacy of the probes was evaluated in detail at cell level and animal level, respectively. Results: The results indicated that MPA and PEG were successfully coupled with APT. APT-based probes were mediated by Mucin 1 into tumor cells, and PEG-modified probe exhibited higher cell affinity. Conclusions: The aptamer-based NIR fluorescent probes are promising candidates for tumor imaging and diagnosis.
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