Journal
MOLECULAR IMAGING AND BIOLOGY
Volume 14, Issue 6, Pages 699-707Publisher
SPRINGER
DOI: 10.1007/s11307-012-0560-4
Keywords
Near infrared fluorescent imaging; Met; ICG-Der-02; Tumor; Early diagnosis
Funding
- Natural Science Foundation Committee of China [NSFC30672015, NSFC30700779, NSFC30800257, NSFC30970776, NSFC81000666, NSFC31050110123, NSFC81071194, NSFC81171395]
- Ministry of Science and Technology [2009ZX09310-004]
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Purpose: A novel near infrared fluorescent probe, l-methyl-methionine (Met)-ICG-Der-02, was synthesized and characterized for in vivo imaging of tumors and early diagnosis of cancers. Method: Met was conjugated with ICG-Der-02 dye through the amide bond function by ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide catalysis chemistry. Met-ICG-Der-02 probe uptake was evaluated on PC3, MDA-MB-231, and human embryonic lung fibroblast cell lines. The dynamics of Met-ICG-Der-02 was investigated in athymic nude mice prior to evaluation of the probe targeting capability in prostate and breast cancer models. Results: Met-ICG-Der-02 was successfully synthesized. Cell experiments demonstrated excellent cellular uptake of Met-ICG-Der-02 on cancer cell lines without cytotoxicity. Optical imaging showed a distinguishable fluorescence signal in the tumor area at 2 h while maximal tumor-to-normal tissue contrast ratio was at 12 h Met-ICG-Der-02 post-injection. Additionally, dynamic study of the probe indicated intestinal and liver-kidney clearance pathways. Conclusion: Met-ICG-Der-02 probe is a promising optical imaging agent for tumor diagnosis, especially in their early stage.
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