Journal
MOLECULAR IMAGING AND BIOLOGY
Volume 14, Issue 3, Pages 315-324Publisher
SPRINGER
DOI: 10.1007/s11307-011-0495-1
Keywords
Vascular disease; Nanoparticles; Inflammation; Angiogenesis; Atherosclerosis; Aneurysms; RGD; Ferritin
Funding
- National Institutes of Health [R21 EB005364, R01 HL078678, P50 HL083800]
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Inflammation and angiogenesis are important contributors to vascular disease. We evaluated imaging both of these biological processes, using Arg-Gly-Asp (RGD)-conjugated human ferritin nanoparticles (HFn), in experimental carotid and abdominal aortic aneurysm (AAA) disease. Macrophage-rich carotid lesions were induced by ligation in hyperlipidemic and diabetic FVB mice ( = 16). AAAs were induced by angiotensin II infusion in apoE(-/-) mice (=10). HFn, with or without RGD peptide, was labeled with Cy5.5 and injected intravenously for near-infrared fluorescence imaging. RGD-HFn showed significantly higher signal than HFn in diseased carotids and AAAs relative to non-diseased regions, both (carotid: 1.88 +/- 0.30 . 1.17 +/- 0.10, = 0.04; AAA: 2.59 +/- 0.24 . 1.82 +/- 0.16, = 0.03) and . Histology showed RGD-HFn colocalized with macrophages in carotids and both macrophages and neoangiogenesis in AAA lesions. RGD-HFn enhances vascular molecular imaging by targeting both vascular inflammation and angiogenesis, and allows more comprehensive detection of high-risk atherosclerotic and aneurysmal vascular diseases.
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