Journal
MOLECULAR IMAGING AND BIOLOGY
Volume 12, Issue 1, Pages 42-53Publisher
SPRINGER
DOI: 10.1007/s11307-009-0240-1
Keywords
Bioluminescence imaging; Luciferase; Beta cell; Pancreatic islet; Transplantation; Optical imaging; Islet mass; Beta cell mass; Imaging
Funding
- Juvenile Diabetes Research Foundation International
- VA Research Service
- National Institutes of Health [DK68764, DK66636, DK69603, DK63439, DK62641, DK068751, T35DK07383, T32EB001628]
- Vanderbilt Mouse Metabolic Phenotyping Center [DK59637]
- Vanderbilt Diabetes Research and Training Center [DK20593]
- SAIRP [U24 CA126588]
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We developed a mouse model that enables non-invasive assessment of changes in beta cell mass. We generated a transgenic mouse expressing luciferase under control of the mouse insulin I promoter [mouse insulin promoter-luciferase-Vanderbilt University (MIP-Luc-VU)] and characterized this model in mice with increased or decreased beta cell mass and after islet transplantation. Streptozotocin-induced, diabetic MIP-Luc-VU mice had a progressive decline in bioluminescence that correlated with a decrease in beta cell mass. MIP-Luc-VU animals fed a high-fat diet displayed a progressive increase in bioluminescence that reflected an increase in beta cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and could be imaged for more than a year. Bioluminescence in the MIP-Luc-VU mouse model is proportional to beta cell mass in the setting of increased and decreased beta cell mass and after transplantation.
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