Journal
MOLECULAR IMAGING AND BIOLOGY
Volume 11, Issue 1, Pages 31-38Publisher
SPRINGER
DOI: 10.1007/s11307-008-0174-z
Keywords
Myocardial infarction; MRI; PET; Stem cell; Rat
Funding
- Medical Research Council (MRC) of Great Britain
- Cancer Research-UK
- MRC [MC_U120081322] Funding Source: UKRI
- Medical Research Council [MC_U120081322] Funding Source: researchfish
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Purpose: The purpose of the study is to track iron-oxide nanoparticle-labelled adult rat bone marrow-derived stem cells (IO-rBMSCs) by magnetic resonance imaging (MRI) and determine their effect in host cardiac tissue using 2-deoxy-2-[F-18]fluoro-D-glucose-positron emission tomography (FDG-PET). Procedures: Infarcted rats were randomised to receive (1) live IO-rBMSCs by direct local injection, or (2) dead IO-rBMSCs as controls; (3) sham-operated rats received live IO-rBMSCs. The rats were then imaged from 2 days to 6 weeks post-cell implantation using both MRI at 9.4T and FDG-PET. Results: Implanted IO-rBMSCs were visible in the heart by MRI for the duration of the study. Histological analysis confirmed that the implanted IO-rBMSCs were present for up to 6 weeks post-implantation. At 1 week post-IO-rBMSC transplantation, PET studies demonstrated an increase in FDG uptake in infarcted regions implanted with live IO-rBMSC compared to controls. Conclusions: Noninvasive multimodality imaging allowed us to visualise IO-rBMSCs and establish their affect on cardiac function in a rat model of myocardial infarction (MI).
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