Journal
MOLECULAR HUMAN REPRODUCTION
Volume 20, Issue 3, Pages 271-281Publisher
OXFORD UNIV PRESS
DOI: 10.1093/molehr/gat081
Keywords
cell culture; cell proliferation; germ cells; animal model
Funding
- National Basic Research Program of China [2013CB967401, 2010CB945001]
- National Nature Science Foundation of China [81370675, 81200472, 81121001]
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Germline stem cell lines possess the abilities of self-renewal and differentiation, and have been established from both mouse and human ovaries. Here, we established a new female germline stem cell (FGSC) line from post-natal rats by immunomagnetic sorting for Fragilis, which showed a normal karyotype, high telomerase activity, and a consistent gene expression pattern of primordial germ cells after 1 year of culture. Using an in vitro differentiation system, the FGSC line could differentiate into oocytes. After liposome-based transfection with green fluorescent protein (GFP) or fat-1 vectors, the FGSCs were transplanted into the ovaries of infertile rats. The transplanted FGSCs underwent oogenesis, and the rats produced offspring carrying the GFP or fat-1 transgene after mating with wild-type male rats. The efficiency of gene transfer was 27.8628.00, and 2 months was needed to produce transgenic rats. These findings have implications in biomedical research and potential applications in biotechnology.
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