Journal
MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
Volume 24, Issue 1, Pages 17-23Publisher
ALLERTON PRESS INC
DOI: 10.3103/S0891416809010042
Keywords
-
Categories
Ask authors/readers for more resources
The aim of this work was to determine the character of the dependence between the GC content of the genes and the level of their usage of preterminal codons (PTCs), i.e., codons that are converted to the terminal codons as a result of single nucleotide substitution. 84 codon usage tables were used as the material, each of which contains average frequencies of codon usage for all encoding sequences belonging to that bacterial specie. Nucleotide sequences encoding for active centers of bacterial adenylate cyclases were used as well. The inverse correlation between the GC content and the total level of PTC usage was observed (R = -0.97). For nucleotide sequences that encode the active centers from class-I adenylate cyclases, the coefficient of correlation between G+C and PTC is -0.75. For sequences that encode for active centers from class-III adenylate cyclases, the coefficient of correlation between G+C and PTC is -0.91. The most of preterminal codons are absolutely GC poor and GC poor relatively to codons synonymous to them. The cause of the inverse correlation between G+C and the level of preterminal codon usage is an increase in the frequencies of their usage due to mutational AT pressure and the decrease of their frequencies of usage due to mutational GC pressure. Evidence was found of the frequent fixation of nonsense mutations in GC poor bacteria. The practical conclusion is as follows: by increasing the GC content of a genome or gene, the probability of a nonsense mutation is decreased.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available