Journal
MOLECULAR ENDOCRINOLOGY
Volume 22, Issue 11, Pages 2433-2447Publisher
ENDOCRINE SOC
DOI: 10.1210/me.2008-0092
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Funding
- ANR [A05056GS]
- HEPADIP [018734]
- Association Nationale de la Recherche Technique
- Ministere de la Recherche
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to the nuclear receptor superfamily and is activated by bile acids such as chenodeoxycholic acid, or synthetic ligands such as GW4064. FXR is implicated in the regulation of bile acid, lipid, and carbohydrate metabolism. Posttranslational modifications regulating its activity have not been investigated yet. Here, we demonstrate that calcium-dependent protein kinase C (PKC) inhibition impairs ligand-mediated regulation of FXR target genes. Moreover, in a transactivation assay, we show that FXR transcriptional activity is modulated by PKC. Furthermore, phorbol 12-myristate 13-acetate, a PKC activator, induces the phosphorylation of endogenous FXR in HepG2 cells and PKC alpha phosphorylates in vitro FXR in its DNA-binding domain on S135 and S154. Mutation of S135 and S154 to alanine residues reduces in cell FXR phosphorylation. In contrast to wild-type FXR, mutant FXRS135AS154A displays an impaired PKC alpha-induced transactivation and a decreased ligand-dependent FXR transactivation. Finally, phosphorylation of FXR by PKC promotes the recruitment of peroxisomal proliferator-activated receptor gamma coactivator 1 alpha. In conclusion, these findings show that the phosphorylation of FXR induced by PKC alpha directly modulates the ability of agonists to activate FXR. (Molecular Endocrinology 22: 2433-2447, 2008)
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