Journal
MOLECULAR ECOLOGY RESOURCES
Volume 11, Issue 3, Pages 567-572Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1755-0998.2010.02955.x
Keywords
16S rRNA gene; MLST; PCR methods; specificity; Wolbachia screening
Funding
- Fundacao para a Ciencia e Tecnologia (FCT) [SFRH/BD/28235/2006]
- CNRS [ATIP]
- ANR Blanc MIRI [BLAN08-1_335497]
- ERC
- Fundação para a Ciência e a Tecnologia [SFRH/BD/28235/2006] Funding Source: FCT
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Wolbachia is a large monophyletic genus of intracellular bacteria, traditionally detected using PCR assays. Its considerable phylogenetic diversity and impact on arthropods and nematodes make it urgent to assess the efficiency of these screening protocols. The sensitivity and range of commonly used PCR primers and of a new set of 16S primers were evaluated on a wide range of hosts and Wolbachia strains. We show that certain primer sets are significantly more efficient than others but that no single protocol can ensure the specific detection of all known Wolbachia infections.
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