Journal
MOLECULAR ECOLOGY RESOURCES
Volume 11, Issue 4, Pages 645-649Publisher
WILEY
DOI: 10.1111/j.1755-0998.2011.03003.x
Keywords
coelomic fluid; DNA extraction; earthworm; Metaphire sieboldi; noninvasive DNA; Oligochaeta
Funding
- Ministry of Education, Science and Culture of Japan
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To investigate whether coelomic fluid secreted by earthworms can be a noninvasive source of DNA, we amplified and sequenced DNA extracted from the coelomic fluid and muscle tissue of eight worms. The sequences obtained using DNA extracted from both sources were identical. All cytochrome c oxidase subunit I (COI) mitochondrial DNA sequences, including those retrieved from GenBank, formed a monophyletic group of Metaphire sieboldi. The results indicate that we successfully extracted total DNA from coelomic fluid secreted by earthworm.
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