Journal
MOLECULAR ECOLOGY RESOURCES
Volume 11, Issue -, Pages 67-70Publisher
WILEY
DOI: 10.1111/j.1755-0998.2010.02978.x
Keywords
association mapping; disease resistance; population genetics; rainbow trout; SNP; steelhead
Funding
- The Danish Agency for Science, Technology and Innovation [2101-08-0017, 09-066097]
- Wild Steelhead Coalition
- Gordon and Betty Moore Foundation
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We establish a TaqMan-based assay panel for genotyping single-nucleotide polymorphisms in rainbow trout and steelhead (Oncorhynchus mykiss). We develop 22 novel single-nucleotide polymorphism markers based on new steelhead sequence data and on assays from sister taxa. Additionally, we adapt 154 previously developed markers to the TaqMan platform. At the beginning of this study, 59 SNPs with TaqMan assays were available to the scientific community. By adding 176 additional TaqMan assays to this number, we greatly expand the biological applications of TaqMan genotyping within both population genetics and quantitative genetics.
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