4.7 Article

Isolation and characterization of microsatellite loci in Symbiodinium B1/B184, the dinoflagellate symbiont of the Caribbean sea fan coral, Gorgonia ventalina

Journal

MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 3, Pages 989-993

Publisher

WILEY
DOI: 10.1111/j.1755-0998.2009.02549.x

Keywords

Gorgonia ventalina; gorgonian coral; microsatellite; population genetics; Symbiodinium; zooxanthellae

Funding

  1. National Science Foundation [OCE-0326705, OCE-0424996]
  2. NSF Graduate Research Fellowship
  3. Theresa Heinz Scholars for Environmental Research
  4. Andrew W. Mellon Foundation

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Here we report primers targeting 10 microsatellite loci of dinoflagellates in the genus Symbiodinium (clade B1/B184) symbiotic with the Caribbean sea fan coral, Gorgonia ventalina. Primers were tested on 12 Symbiodinium B1/B184 cultures, as well as 40 genomic DNA extracts of G. ventalina tissue samples. All loci were polymorphic with allelic richness ranging from 4-16. Gene diversity ranged from 0.15 to 0.91. These primers provide powerful tools for examining the fine-scale population structure and dynamics of Symbiodinium within a single host species.

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