4.7 Article

Microsatellite primers for the four Galapagos mockingbird species (Mimus parvulus, Mimus macdonaldi, Mimus melanotis and Mimus trifasciatus)

Journal

MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 6, Pages 1538-1541

Publisher

WILEY
DOI: 10.1111/j.1755-0998.2009.02704.x

Keywords

Mimus; multiplex PCR; Nesomimus

Funding

  1. Zoological Museum
  2. Forschungskredit University of Zurich
  3. Basler Stiftung fur biologische Forschung

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Nineteen di- and tetranucleotide and one trinucleotide microsatellite DNA markers were isolated from the Galapagos mockingbird (Mimus parvulus) and tested for cross-species amplification in the other three mockingbird species in the Galapagos. In addition, primers for two microsatellite loci previously developed for Mimus polyglottos were redesigned to obtain shorter amplification fragments. The number of alleles per locus and species ranged from 1 to 8, and expected heterozygosity varied from 0.0 to 0.809. These microsatellite markers will be useful to study levels of inbreeding in different island populations.

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