Journal
MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 3, Pages 923-925Publisher
WILEY
DOI: 10.1111/j.1755-0998.2008.02486.x
Keywords
ISSR; Melipona; microsatellite; population genetics; stingless bees
Funding
- Brazilian agencies FAPEMIG [EDT 245/05]
- CNPq
- UFV
- CAPES
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Eight microsatellite primers were developed from ISSR (intersimple sequence repeats) markers for the stingless bee Melipona rufiventris. These primers were tested in 20 M. rufiventris workers, representing a single population from Minas Gerais state. The number of alleles per locus ranged from 2 to 5 (mean = 2.63) and the observed and expected heterozygosity values ranged from 0.00 to 0.44 (mean = 0.20) and from 0.05 to 0.68 (mean = 0.31), respectively. Several loci were also polymorphic in M. quadrifasciata, M. bicolor, M. mandacaia and Partamona helleri and should prove useful in population studies of other stingless bees.
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