4.7 Article

Isolation of microsatellite loci from expressed sequence tag library of Puccinia striiformis f. sp tritici

Journal

MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 1, Pages 236-238

Publisher

WILEY
DOI: 10.1111/j.1755-0998.2008.02423.x

Keywords

expressed sequence tags; microsatellite; Puccinia striiformis; wheat yellow rust

Funding

  1. National Basic Research Program of China [200611D1002 03]
  2. Cultivation Fund of the Key Scientific and Technical Innovation Project
  3. Ministry of Education of China [2005295]
  4. Nature Science Foundation of China [30671350]
  5. [200558]
  6. [B07049]

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We described twenty polymorphic microsatellite loci derived from the expressed sequence tags of Puccinia striiformis f. sp. tritici, which causes yellow rust disease on wheat. The numbers of alleles range from two to six and eight microsatellite loci show significant similarities to known genes. Observed and expected heterozygosities ranged from 0.12 to 0.78 and from 0.24 to 0.87, respectively.

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