Journal
MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 1, Pages 339-343Publisher
WILEY
DOI: 10.1111/j.1755-0998.2008.02407.x
Keywords
ancestry-informative markers; hybridization; Oncorhynchus mykiss; single nucleotide polymorphism; SNP
Funding
- California Department of Fish and Game and US Fish and Wildlife Service [P0480052, P0380035]
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We characterize 20 single nucleotide polymorphism assays for evaluating hybridization between native golden trout subspecies (Oncorhynchus mykiss aguabonita and O. m. whitei) and introduced rainbow trout strains. These assays utilize the 5'-nuclease reaction, facilitating high-throughput genotyping of many individuals and making them useful in quantifying and monitoring introgression and potentially applicable to studies of other O. mykiss groups. Minor allele frequency differentials (delta q) among native and introduced rainbow groups ranged from 0 to 1, with an average differential of 0.75 for both subspecies aguabonita and whitei relative to the hatchery rainbow trout strain.
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