Journal
MOLECULAR ECOLOGY RESOURCES
Volume 9, Issue 3, Pages 777-783Publisher
WILEY
DOI: 10.1111/j.1755-0998.2008.02320.x
Keywords
angiosperm; cpDNA; cpSSRs; microsatellites; noncoding chloroplast DNA; simple sequence repeats; universal primers
Funding
- Australian Research Council (ARC) [DP0451374]
- Australian Research Council [DP0451374] Funding Source: Australian Research Council
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We present a new set of universal de novo sequencing primers targeting noncoding chloroplast DNA. The set of 107 polymerase chain reaction (PCR) primers span approximately 86% of the noncoding nucleotides in the large single copy region of Nicotiana tabacum, Oryza sativa and the orchid Phalaenopsis aphrodite. PCR tests confirmed the primers are effective in a wide range of monocots and dicots. More than 19.5 kb of cpDNA sequence was obtained across representative orchid genera with up to 82 chloroplast simple sequence repeats (cpSSRs) detected per genus. This primer set will facilitate both phylogenetic studies and rapid discovery of cpSSRs for plants, such as orchids, where there are limited genomic resources.
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