Journal
MOLECULAR ECOLOGY RESOURCES
Volume 8, Issue 6, Pages 1189-1201Publisher
WILEY
DOI: 10.1111/j.1755-0998.2008.02297.x
Keywords
Aphididae; COI; DNA barcoding; mitochondrial DNA; parthenogenesis; species identification
Funding
- Agriculture and Agri-Food Canada
- NSERC
- Genome Canada
- Gordon and Betty Moore Foundation
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A 658-bp fragment of mitochondrial DNA from the 5 ' region of the mitochondrial cytochrome c oxidase 1 (COI) gene has been adopted as the standard DNA barcode region for animal life. In this study, we test its effectiveness in the discrimination of over 300 species of aphids from more than 130 genera. Most (96%) species were well differentiated, and sequence variation within species was low, averaging just 0.2%. Despite the complex life cycles and parthenogenetic reproduction of aphids, DNA barcodes are an effective tool for identification.
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