Journal
MOLECULAR ECOLOGY RESOURCES
Volume 8, Issue 6, Pages 1285-1287Publisher
BLACKWELL PUBLISHING
DOI: 10.1111/j.1755-0998.2008.02356.x
Keywords
mayfly; microsatellite enrichment; PCR
Funding
- Studienstiftung des deutschen Volkes
- Grainger Foundation
- Pritzker Foundation
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We describe the isolation of ten polymorphic microsatellite loci from the mayfly Ameletus inopinatus. Loci had di- or trinucleotide repeat motifs and were highly variable with three to 17 alleles (mean = 7.15). Observed heterozygosity ranged from 0.143 to 0.905. One locus (Ami_202) showed significant deviation from Hardy-Weinberg equilibrium in one population, but no evidence for null alleles. One locus (Ami_73) was significantly linked with three other loci. The remaining nine loci should prove highly informative for population genetic studies.
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