Journal
MOLECULAR ECOLOGY
Volume 23, Issue 24, Pages 5943-5946Publisher
WILEY
DOI: 10.1111/mec.12964
Keywords
allele dropout; genotyping error; next generation sequencing; PCR duplicates; pooling; population genomics
Funding
- Direct For Biological Sciences
- Division Of Environmental Biology [1258203] Funding Source: National Science Foundation
- Division Of Environmental Biology
- Direct For Biological Sciences [1067613] Funding Source: National Science Foundation
- NIGMS NIH HHS [P30GM103324] Funding Source: Medline
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Puritz etal. provide a review of several RADseq methodological approaches in response to our Population Genomic Data Analysis' workshop (Sept 2013) review (Andrews & Luikart 2014). We agree with Puritz etal. on the importance for researchers to thoroughly understand RADseq library preparation and data analysis when choosing an approach for answering their research questions. Some of us are currently using multiple RADseq protocols, and we agree that the different methods may offer advantages in different cases. Our workshop review did not intend to provide a thorough review of RADseq because the workshop covered a broad range of topics within the field of population genomics. Similarly, neither the response of Puritz etal. nor our comments here provide sufficient space to thoroughly review RADseq. Nonetheless, here we address some key points that we find unclear or potentially misleading in their evaluation of techniques.
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