Journal
MOLECULAR CELL
Volume 53, Issue 2, Pages 290-300Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2013.11.012
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Funding
- National Institutes of Health [GM-64844, R37-37120]
- Howard Hughes Medical Institute
- Helen Hay Whitney Foundation
- Helen L. and Martin S. Kimmel Center for Stem Cell Biology
- CONACyT [213029]
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JARID2 is an accessory component of Polycomb repressive complex-2 (PRC2) required for the differentiation of embryonic stem cells (ESCs). A role for JARID2 in the recruitment of PRC2 to target genes silenced during differentiation has been put forward, but the molecular details remain unclear. We identified a 30-amino-acid region of JARID2 that mediates interactions with long noncoding RNAs (IncRNAs) and found that the presence of IncRNAs stimulated JARID2-EZH2 interactions in vitro and JARID2-mediated recruitment of PRC2 to chromatin in vivo. Native and crosslinked RNA immunoprecipitations of JARID2 revealed that Meg3 and other IncRNAs from the imprinted Dlk1-Dio3 locus, an important regulator of development, interacted with PRC2 via JARID2. Lack of MEG3 expression in human induced pluripotent cells altered the chromatin distribution of JARID2, PRC2, and H3K27me3. Our findings show that IncRNAs facilitate JARID2-PRC2 interactions on chromatin and suggest a mechanism by which IncRNAs contribute to PRC2 recruitment.
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