Journal
MOLECULAR CELL
Volume 50, Issue 4, Pages 552-564Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2013.04.029
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Funding
- NCI Grant [U54CA143803]
- Chambers Medical Foundation
- Cancer Center Grant [P30 CA014195]
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Cellular stress results in profound changes in RNA and protein synthesis. How cells integrate this intrinsic, p53-centered program with extracellular signals is largely unknown. We demonstrate that TGF-beta 1 signaling interferes with the stress response through coordinate transcriptional and translational repression of p53 levels, which reduces p53-activated transcription, and apoptosis in precancerous cells. Mechanistically, E2F-4 binds constitutively to the TP53 gene and induces transcription. TGF-beta 1-activated Smads are recruited to a composite Smad/E2F-4 element by an E2F-4/p107 complex that switches to a Smad corepressor, which represses TP53 transcription. TGF-beta 1 also causes dissociation of ribosomal protein RPL26 and elongation factor eEF1A from p53 mRNA, thereby reducing p53 mRNA association with polyribosomes and p53 translation. TGF-beta 1 signaling is dominant over stress-induced transcription and translation of p53 and prevents stress-imposed downregulation of Smad proteins. Thus, crosstalk between the TGF-beta and p53 pathways defines a major node of regulation in the cellular stress response, enhancing drug resistance.
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