Journal
MOLECULAR CELL
Volume 43, Issue 4, Pages 528-539Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2011.06.025
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Funding
- Burroughs Wellcome Fund
- National Institutes of Health [GM092925]
- National Science Foundation [1054025]
- Hellman Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1054025] Funding Source: National Science Foundation
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The ability to specify and maintain discrete cell fates is essential for development. However, the dynamics underlying selection and stability of distinct cell types remain poorly understood. Here, we provide a quantitative single-cell analysis of commitment dynamics during the mating-mitosis switch in budding yeast. Commitment to division corresponds precisely to activating the G1 cyclin positive feedback loop in competition with the cyclin inhibitor Fan. Cyclin-dependent phosphorylation and inhibition of the mating pathway scaffold Ste5 are required to ensure exclusive expression of the mitotic transcriptional program after cell cycle commitment. Failure to commit exclusively results in coexpression of both cell cycle and pheromone-induced genes, and a morphologically mixed inviable cell fate. Thus, specification and maintenance of a cellular state are performed by distinct interactions, which are likely a consequence of disparate reaction rates and may be a general feature of the interlinked regulatory networks responsible for selecting cell fates.
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