Journal
MOLECULAR CELL
Volume 41, Issue 1, Pages 21-32Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2010.12.007
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Funding
- Wellcome Trust
- Spanish Ministry of Science and Innovation [BFU2006-05260, CSD2007-0015]
- Junta de Andalucia [BIO102, CV2549]
- ESF
- B.I.F. studentship
- European Molecular Biology Organization (EMBO)
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Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses a wider function by restricting the occurrence of RNA:DNA hybrids that may naturally form during transcription, when nascent RNA hybridizes to DNA prior to its packaging into RNA protein complexes. These hybrids displace the nontranscribed strand and create R loop structures. Loss of Sen1 results in transient R loop accumulation and so elicits transcription-associated recombination. SEN1 genetically interacts with DNA repair genes, suggesting that R loop resolution requires proteins involved in homologous recombination. Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Semi is to prevent their accumulation and associated genome instability.
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