Journal
MOLECULAR CELL
Volume 37, Issue 5, Pages 714-727Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2010.02.009
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Funding
- Japan Science and Technology Agency (JST)
- Japan Society for the Promotion of Science [20810021]
- YASUDA Medical Foundation
- Uehara Memorial Foundation
- Takeda Science Foundation
- Yamada Apiculture Center Inc
- Great Britain Sasakawa Foundation
- Ministry of Education, Culture, Sports, Sciences and Technology of Japan
- Medical Research Council
- EC-RTN
- Grants-in-Aid for Scientific Research [20810021] Funding Source: KAKEN
- Medical Research Council [G0501450, G0801130B] Funding Source: researchfish
- MRC [G0501450] Funding Source: UKRI
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Nucleotide excision repair (NER) is the most versatile DNA repair system that deals with the major UV photoproducts in DNA, as well as many other DNA adducts. The early steps of NER are well understood, whereas the later steps of repair synthesis and ligation are not. In particular, which polymerases are definitely involved in repair synthesis and how they are recruited to the damaged sites has not yet been established. We report that, in human fibroblasts, approximately half of the repair synthesis requires both pol kappa and pol delta, and both polymerases can be recovered in the same repair complexes. Pol kappa is recruited to repair sites by ubiquitinated PCNA and XRCC1 and pol delta by the classical replication factor complex RFC1-RFC, together with a polymerase accessory factor, p66, and unmodified PCNA. The remaining repair synthesis is dependent on pol epsilon, recruitment of which is dependent on the alternative clamp loader CTF18-RFC.
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