Journal
MOLECULAR CELL
Volume 38, Issue 2, Pages 202-210Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2010.02.026
Keywords
-
Categories
Funding
- EMBO
- Cancer Research UK
- Association of International Cancer Research
- European Community [LSHG-CT-2005-512113]
- Cancer Research UK [11567] Funding Source: researchfish
- Worldwide Cancer Research [09-0730] Funding Source: researchfish
Ask authors/readers for more resources
During transcript elongation in vitro, backtracking of RNA polymerase II (RNAPII) is a frequent occurrence that can lead to transcriptional arrest. The polymerase active site can cleave the transcript during such backtracking, allowing transcription to resume. Transcript cleavage is either stimulated by elongation factor TFIIS or occurs much more slowly in its absence. However, whether backtracking actually occurs in vivo, and whether transcript cleavage is important to escape it, has been unclear. Using a yeast TFIIS mutant that lacks transcript cleavage stimulatory activity and simultaneously inhibits unstimulated cleavage, we now provide evidence that escape from backtracking via transcript cleavage is essential for cell viability and efficient transcript elongation. Our results suggest that transcription problems leading to backtracking are frequent in vivo and that reactivation of backtracked RNAPII is crucial for transcription.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available