Journal
MOLECULAR CELL
Volume 40, Issue 6, Pages 988-1000Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2010.11.016
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Funding
- National Institutes for Health [GM041784, GM054099]
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Holliday junction (HJ) resolution is required for segregation of chromosomes and for formation of crossovers during homologous recombination. The identity of the resolvase(s) that functions in vivo has yet to be established, although several proteins able to cut HJs in vitro have been identified as candidates in yeasts and mammals. Using an assay to detect unselected products of mitotic recombination, we found a significant decrease in crossovers in the Saccharomyces cerevisiae mus81 Delta mutant. Yen1 serves a backup function responsible for resolving intermediates in mus81 Delta mutants, or when conversion tracts are short. In the absence of both Mus81 and Yen1, intermediates are not channeled exclusively to noncrossover recombinants, but instead are processed by Pol32-dependent break-induced replication (BIR). The channeling of recombination from reciprocal exchange to BIR results in greatly increased spontaneous loss of heterozygosity (LOH) and chromosome mis-segregation in the mus81 Delta yen1 Delta mutant, typical of the genomic instability found in tumor cells.
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