Journal
MOLECULAR CELL
Volume 33, Issue 3, Pages 299-311Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2009.01.019
Keywords
-
Categories
Funding
- AICR
- EMBO-LTF
- MRC UK
- MRC studentship
- MRC Strategic Grant [G0301153]
- Ministerio de Educacion y Ciencia, Spain
- EC Marie-Curie Excellence [MEXT-CT-014171]
- Wellcome Trust [065061/Z]
- NIH [RO1 GM068676]
- Wellcome Trust Principal Research Fellow
- Medical Research Council [G0301153] Funding Source: researchfish
- MRC [G0301153] Funding Source: UKRI
Ask authors/readers for more resources
The mechanisms ensuring specific incorporation of CENP-A at centromeres are poorly understood. Mis16 and Mis18 are required for CENP-A localization at centromeres and form a complex that is conserved from fission yeast to human. Fission yeast sim1 mutants that alleviate kinetochore domain silencing are defective in Scm3(Sp), the ortholog of budding yeast Scm3(Sc). Scm3(Sp) depends on Mis16/18 for its centromere localization and like them is recruited to centromeres in late anaphase. Importantly, Scm3(Sp) coaffinity purifies with CENP-A(Cnp1) and associates with CENP-A(Cnp1) in vitro, yet localizes independently of intact CENP-A(Cnp1) chromatin and is differentially released from chromatin. While Scm3(Sc) has been proposed to form a unique hexameric nucleosome with CENP-A(Cse4) and histone H4 at budding yeast point centromeres, we favor a model in which Scm3(Sp) acts as a CENP-A(Cnp1) receptor/assembly factor, cooperating with Mis16 and Mis18 to receive CENP-A(Cnp1) from the Sim3 escort and mediate assembly of CENP-A(Cnp1) into subkinetochore chromatin.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available