Journal
MOLECULAR CELL
Volume 29, Issue 2, Pages 180-190Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2007.11.021
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Funding
- NIGMS NIH HHS [R01 GM032734, R01 GM061576-07, GM 61576, R01 GM061576, GM 32734] Funding Source: Medline
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The mechanism of translation in eubacteria and organelles is thought to be similar. In eubacteria, the three initiation factors IF1, IF2, and IF3 are vital. Although the homologs of IF2 and IF3 are found in mammalian mitochondria, an IF1 homolog has never been detected. Here, we show that bovine mitochondrial IF2 (IF2(mt)) complements E coli containing a deletion of the IF2 gene (E coli Delta infB). We find that IF1 is no longer essential in an IF2(mt)-supported E. coli Delta infB strain. Furthermore, biochemical and molecular modeling data show that a conserved insertion of 37 amino acids in the IF2(mt) substitutes for the function of IF1. Deletion of this insertion from IF2(mt) supports E. coli for the essential function of IF2. However, in this background, IF1 remains essential. These observations provide strong evidence that a single factor (IF2(mt)) in mammalian mitochondria performs the functions of two eubacterial factors, IF1 and IF2.
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