Journal
MOLECULAR CELL
Volume 32, Issue 1, Pages 96-105Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2008.09.009
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Funding
- Howard Hughes Medical Institute Funding Source: Medline
- NCI NIH HHS [R37 CA025417-30, R37 CA025417-29, R37 CA025417] Funding Source: Medline
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Skeletal muscle differentiation requires a cascade of transcriptional events to control the spatial and temporal expression of muscle-specific genes. Until recently, muscle-specific transcription was primarily attributed to prototypic enhancer-binding factors, while the role of core promoter recognition complexes in directing myogenesis remained unknown. Here, we report the development of a purified reconstituted system to analyze the properties of a TAF3/TRF3 complex in directing transcription initiation at the Myogenin promoter. Importantly, this new complex is required to replace the canonical TRID to recapitulate MyoD-dependent activation of Myogenin. In vitro and cell-based assays identify a domain of TAF3 that mediates coactivator functions targeted by MyoD. Our findings also suggest changes to CRSP/Mediator in terminally differentiated myotubes. This switching of the core promoter recognition complex during myogenesis allows a more balanced division of labor between activators and TAF coactivators, thus providing another strategy to accommodate cell-specific regulation during metazoan development.
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