Journal
MOLECULAR CELL
Volume 30, Issue 1, Pages 98-107Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2008.02.016
Keywords
-
Categories
Funding
- Intramural NIH HHS [Z01 HD001009-15, Z99 HD999999] Funding Source: Medline
- NIGMS NIH HHS [R01 GM046226-13S1, R01 GM046226-10A1, R01 GM046226-12, R01 GM046226-11, R01 GM046226-09, R01 GM046226, R01 GM046226-13] Funding Source: Medline
Ask authors/readers for more resources
The LTR-retrotransposon Tf1 preserves the coding capacity of its host Schizosaccharomyces pombe by integrating upstream of open reading frames (ORFs). To determine which features of the target sites were recognized by the transposon, we introduced plasmids containing candidate insertion sites into S. pombe and mapped the positions of integration. We found that Tf1 was targeted specifically to the promoters of Pol II-transcribed genes. A detailed analysis of integration in plasmids that contained either ade6 or fbp 1 revealed insertions occurred in the promoters at positions where transcription factors bound. Further experiments revealed that the activator AM p and its binding site were required for directing integration to the promoter of fbp 1. An interaction between Tf1 integrase and AM p was observed, indicating that integration at fbp1 was mediated by the activator bound to its promoter. Surprisingly, we found Tf1 contained sequences that activated transcription, and these substituted for elements of the ade6 promoter disrupted by integration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available