Association of the miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G Polymorphisms With Gastric Cancer Risk and Survival in the Korean Population

We investigated whether four common microRNA polymorphisms (miR-146aC>G [rs2910164], miR-149T>C [rs2292832], miR-196a2T>C [rs11614913], and miR-499A>G [rs3746444]) are associated with the susceptibility and prognosis of gastric cancer in the Korean population. The four microRNA single-nucleotide polymorphisms (SNPs) were identified in a case-control study (461 patients; 447 controls) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in the Korean population. When patients were stratified into diffuse and intestinal-type gastric cancer groups, subjects with the miR-499AG and AG+GG genotypes had reduced adjusted odds ratios (AORs) for diffuse-type gastric cancer (AOR=0.54 with 95% confidence interval [CI]=0.31-0.97; AOR=0.57 with 95% CI=0.33-0.97). In the stratified analyses for gastric cancer risk, the miR-146aGG and CG+GG genotypes were associated with increased risk of gastric cancers among the non-smokers, whereas the miR-149TC and TC+CC genotypes showed lower risk of gastric cancer in males. The miR-196a2CC genotype was associated with elevated gastric cancer risk among females. For gastric cancer prognosis, intestinal-type gastric cancer patients with miR-146aCG+GG genotypes had significantly higher survival rates (log-rank P=0.030) than patients with the CC genotype, and patients with the miR-499AA genotype had significantly increased survival rates compared to patients with the AG+GG genotypes (log-rank P=0.013). When miR-146aCG+GG and miR-499AA genotypes were combined, the survival rate of intestinal-type gastric cancer patients was elevated (log-rank P<0.001). No association was found between gastric or diffuse-type cancer prognosis and other miRNAs. Our data demonstrate that specific miRNA SNPs are associated with gastric cancer susceptibility (miR-499A>G) and prognosis (miR-146aC>G and miR-499A>G) in the Korean population depending on gastric cancer type. (c) 2012 Wiley Periodicals, Inc.